Two-Dimensional Gel Electrophoresis (2D-GE) is a powerful technique that separates proteins in two distinct steps for high-resolution analysis. In the first dimension, proteins are separated based on their isoelectric points using isoelectric focusing (IEF). In the second dimension, the focused proteins are further separated by their molecular weight using SDS-PAGE. This combination allows precise separation of complex protein mixtures, making 2D-GE a cornerstone method in proteomics for studying protein expression, isoforms, and post-translational modifications.