Multiplexed and Automated Isoelectric Focusing (IEF) Systems are advanced platforms designed to increase throughput and reproducibility in protein separation. Multiplexed systems allow simultaneous processing of multiple samples in parallel, greatly speeding up analysis times for large sample sets. Automation integrates steps like sample loading, focusing, mobilization, and detection, reducing manual intervention and variability. Together, these innovations enable high throughput, reliable, and user friendly workflows ideal for clinical diagnostics, biopharmaceutical quality control, and large scale proteomics studies.